The national toll-free hotline - 800 746 1553 - can be used not only to report serious accidents, injuries or illness that occur on site, but can also be utilised to make the agency aware of hazards at mines.
The campaign began last summer and, as part of the effort, MSHA will distribute promotional materials during inspections.
Agency head Richard Stickler said the centralised reporting line makes it easier for anyone to make contact.
"We want to remind miners, also, of their right to confidentially and anonymously report to MSHA any uncorrected hazardous workplace conditions they may encounter," said Stickler.
"We can better prevent mining injuries and fatalities when hazards are quickly removed from the work environment."
Last year's MINER Act included a regulation requiring all accidents be reported to MSHA within 15 minutes of realisation, with violators facing a $US5000 to $60,000 civil penalty.
Miners needing extra "One Call Does It All" materials for their operations can contact the National Mine Health and Safety Academy in Beckley, West Virginia.
