The funding will be used for 20 projects across 15 regional communities.
Deputy premier Steven Miles said Queensland communities should be the first to benefit when the resource sector they supported succeeded.
"That's why the government has partnered with the resources sector to deliver the $100 million fund," he said.
"The Royal Flying Doctor Service will receive a new aeromedical base, co-located with a new Lifeflight base, in Mount Isa so they can continue to deliver their excellent services for our most remote communities and establish Mount Isa as a major aeromedical hub .
"Childcare and early learning services will receive a boost with a new facility in Cloncurry and a new services hub in Julia Creek.
"We'll improve regional Queenslanders' already great lifestyle with an upgrade to the Springsure Aquatic Centre, the Biloela Splash Park, and a Mount Isa aqua play facility."
Resources minister Scott Stewart said the RCIF was a voluntary partnership between the Queensland government, resource companies, and the Queensland Resources Council.
"The Queensland government has committed $30 million to the fund with participating Queensland coal and mineral resources companies contributing $70 million," he said.
"The projects not only deliver infrastructure that further improves liveability in resource communities, they also create more jobs and more opportunities for regional Queenslanders.
"The resources industry helps support good jobs in our regions so it's great to these resources communities benefiting from this initiative."
Queensland Resources Council CEO Ian Macfarlane said: "We are very proud that members of our industry are contributing a total of $70 million to the RCIF for projects that will have significant benefits for our local resource communities".
An expert advisory committee considered RCIF applications for both funding rounds.
Committee chairman Tony Mooney said there had been strong interest in both RCIF rounds.
"Resources communities can now get ready to continue benefiting from the RCIF through this new round of projects," Mooney said.
"The committee looks forward to resources communities, including workers and their families, enjoying the many benefits of these projects."