About 15 protestors camped on the line and blocked it for two nights starting on Monday.
Led by Whitsunday Councillor Peter Ramage, the protestors were upset with the level of coal train traffic and emissions.
But QRN met with the protestors and ended the blockade yesterday.
“QR National has committed to working with the Collinsville community to lessen the impact of coal trains in the area, including looking at current operations with a view to decreasing the frequency of trains stopping in the town,” a QRN spokesperson told ILN.
“We have also committed to retesting noise, dust and vibration levels with involvement from the community.
“These matters will be further discussed at the regular meetings to be held between QR National and representatives of the Collinsville community.”